Fluorescent-Antibody Test for Detection of Antibodies to Rabies Virus in Human Sera
نویسنده
چکیده
The correlation between the modified counterimmunoelectrophoresis test and the indirect fluorescent-antibody test for detecting rabies virus antibodies in human sera was studied, using as a reference the standard mouse neutralization test. Higher sensitivity was observed with the counterimmunoelectrophoresis technique than with the indirect fluorescent-antibody method. The counterimmunoelectrophoresis test provides a practical quantitative alternative to the indirect fluorescent-antibody method.
منابع مشابه
Comparison between a modified counterimmunoelectrophoresis test and the indirect fluorescent-antibody test for detection of antibodies to rabies virus in human sera.
The correlation between the modified counterimmunoelectrophoresis test and the indirect fluorescent-antibody test for detecting rabies virus antibodies in human sera was studied, using as a reference the standard mouse neutralization test. Higher sensitivity was observed with the counterimmunoelectrophoresis technique than with the indirect fluorescent-antibody method. The counterimmunoelectrop...
متن کاملVaccination against rabies and protective antibodies - comparison of ELISA and fluorescent antibody virus neutralization (FAVN) assays
The aim of the study was monitoring the efficacy of primary vaccination against rabies and the need for booster doses. These studies validate at the same time recent technological improvements in laboratory diagnostics of the level of rabies protection in human sera. Research was carried out into the level of antibodies, considering that an antibody titer ≥0.5 IU/mL is protective. We used Plate...
متن کاملComparison of the detection and quantification of rabies antibodies in canine sera
Rabies antibodies in canine sera were detected and quantified by methods: virus neutralisation test on mice (VNT), rapid fluorescence focus inhibition test (RFFIT), and fluorescent antibody virus neutralisation (FAVN). e results of rabies antibodies levels in non-vaccinated dogs obtained by all three methods were in correlation. e comparison of rabies antibody titres determined in vaccinated ...
متن کاملDevelopment of a Western Blot Assay for Detection of Antibodies against HSV Using Purified HSV Virions Prepared by Sucrose Density Gradient
Objective(s) Herpes simplex viruses (HSVs) have widespread and ubiquitous prevalence in the human population and they have received a great deal of attention due to the range of diseases, they caused as a result of an infection. It seems that the fast and reliable diagnostic methods are needed for detecting the herpes simplex virus type 1 (HSV1) antibodies especially in patients with HSV encep...
متن کاملGeneration of Recombinant Rabies Virus CVS-11 Expressing eGFP Applied to the Rapid Virus Neutralization Test
The determination of levels of rabies virus-neutralizing antibody (VNA) provides the foundation for the quantitative evaluation of immunity effects. The traditional fluorescent antibody virus neutralization test (FAVN) using a challenge virus standard (CVS)-11 strain as a detection antigen and staining infected cells with a fluorescein isothiocyanate (FITC)-labeled monoclonal antibody, is expen...
متن کامل